- Open Access
- Open Peer Review
Virginity testing: a systematic review
© The Author(s). 2017
- Received: 20 October 2016
- Accepted: 28 April 2017
- Published: 18 May 2017
So-called virginity testing, also referred to as hymen, two-finger, or per vaginal examination, is the inspection of the female genitalia to assess if the examinee has had or has been habituated to sexual intercourse. This paper is the first systematic review of available evidence on the medical utility of virginity testing by hymen examination and its potential impacts on the examinee.
Ten electronic databases and other sources for articles published in English were systematically searched from database inception until January 2017. Studies reporting on the medical utility or impact on the examinee of virginity testing were included. Evidence was summarized and assessed via a predesigned data abstraction form. Meta-analysis was not possible.
Seventeen of 1269 identified studies were included. Summary measures could not be computed due to study heterogeneity. Included studies found that hymen examination does not accurately or reliably predict virginity status. In addition, included studies reported that virginity testing could cause physical, psychological, and social harms to the examinee.
Despite the lack of evidence of medical utility and the potential harms, health professionals in multiple settings continue to practice virginity testing, including when assessing for sexual assault. Health professionals must be better informed and medical and other textbooks updated to reflect current medical knowledge. Countries should review their policies and move towards a banning of virginity testing.
- Virginity testing
- Gynecological examination
Virginity testing is a practice some communities use to detect which women or girls are ‘virgins’ (i.e. have not had sexual intercourse). People have different ways of trying to detect who are virgins. Some think you can tell by looking at the hymen (a piece of tissue that covers the vagina), while others think you can tell by looking at the size of the vagina. Communities often use the test to separate “pure” females from “impure” females. In some communities, only the “pure” females are to be married, have certain jobs, or be respected. This review searched ten different databases, and found 17 reports on virginity testing. We studied whether looking at the hymen can determine who is a ‘virgin’, and how the exam affects the girl or woman being tested. Our review found that virginity testing is not good at detecting who has not had sexual intercourse, and that it can hurt the person being tested – physically, mentally, and socially. Our hope is to make more people and countries aware of this to prevent harm to women and girls.
So-called virginity testing, also referred to as hymen, two-finger, or per vaginal examination, is the inspection of the female genitalia to determine if the individual has had or has been habituated to sexual intercourse . The two most common techniques are inspection of the hymen for size or tears, and two-finger vaginal insertion to measure size of the introitus or laxity of the vaginal wall. Both techniques are performed under the belief that there is a specific appearance of genitalia that demonstrates habituation to sexual intercourse [1, 2]. The prevailing social rationale for testing is that an unmarried female’s virginity is indicative of her moral character and social value, whether in the context of marriage eligibility, sexual assault assessment, employment application, or otherwise [1, 2].
Virginity examinations are most commonly performed on unmarried females, often without consent or in situations where individuals are unable to give consent . Depending on the region, the examiner may be a medical doctor, police officer, or community leader. Countries where this practice has been reported include Afghanistan, Bangladesh, Egypt, India, Indonesia, Iran, Jordan, Palestine, South Africa, Sri Lanka, Swaziland, Turkey, and Uganda [3–14]. Virginity testing is performed in various countries for reasons that vary by region. Certain communities in rural KwaZulu Natal in South Africa and Swaziland have performed virginity tests on school-aged girls with the aim to deter pre-marital sexual activity and reduce HIV prevalence [3, 4]. In India, the test has been part of the sexual assault assessment of female rape victims . In Indonesia, the exam has been part of the application process for women to join the Indonesian police force [12, 13]. Due to increased globalization, reports of virginity testing are appearing in countries with no prior history, including Canada, Spain, Sweden, and the Netherlands . Despite it being a long-standing tradition in some communities, formal assessments of the frequency of virginity testing are scarce. Thus, prevalence cannot be accurately described; however, anecdotes of its incidence occur in a variety of social settings in different countries.
The growing attention to eliminating sexual violence has raised awareness of the routine use of virginity testing in some settings . This study was undertaken to systematically review all available published studies on virginity testing to determine its medical relevance and its impacts on the examinee. Ultimately, this review will inform the World Health Organization’s recommendations regarding virginity testing.
The study population of interest was females who underwent any type of ‘virginity test’ and/or hymen examination. We did not enforce limitations on age, race/ethnicity, nationality, or other participant characteristics. Outcomes that were of interest in determining medical utility included physical exam findings of the female hymen (such as hymenal tear, perforation, or size of opening) that could indicate vaginal penetration, and the examiner’s ability to accurately and/or reliably identify hymenal features by physical exam. Outcomes that were of interest in determining impact on the examinee included personal or close-contact accounts of the effects of the virginity test on the examinee’s well-being (such as physical, psychological, and social consequences).
Two reviewers (Olson and García-Moreno) independently screened titles and abstracts and selected relevant studies for full text analysis. References of relevant articles were screened to find additional studies. RO then performed full text assessments, extracted data, and, in consultation with CGM, made decisions about study inclusion and exclusion. Any differences in opinion in the screening process, data extraction and in analysis were resolved through re-examination of the study and further discussion. If agreement had not been reached, the reviewers would have consulted a third reviewer.
Data were extracted using predesigned data extraction forms. The forms contained questions regarding study type, participant characteristics, role of examiner, method of examination, and outcomes measured. Data extracted from studies reporting on the impact of virginity testing on the examinee was synthesized with a thematic synthesis approach informed by the Cochrane Collaboration guidelines . A spreadsheet was created of all the data extracted from these studies, and thematic analysis methods were used to develop broad themes.
The quality of each study was assessed using the grading system of the United States Preventive Services Task Force (USPSTF) [19, 20]. This grading system examines both study design and the internal validity of each study. (Additional information regarding the USPSTF grading criteria is provided in the Additional file 1). Internal validity measures how well the study was conducted, and a level of good, fair, or poor is assigned. Due to the lack of available research and presence of heterogeneity with respect to study design and aims, measures, and outcomes, a structured synthesis was undertaken, rather than a metaanalysis .
Summary of included studies reporting on medical utility
Study design and population
Quality of evidence
Berenson et al. 2000 
Case-control study at two centers in United States (n = 392)
Examiner(s): 1–2 physicians
Age of examinees: 3–8 years
Group 1: controls (n = 200)
Group 2: females with history of penetration (n = 192)
2.5% of Group 2 had physical findings that differed from those found in Group 1
Only one hymenal feature difference was found between the two groups; a septate hymen was observed more often in Group 2 than Group 1 (4% vs 1%; P = .03)
Kellog et al. 2004 
Retrospective case review at one center in United States (n = 36)
Examiner(s): 1 physician, 2 nurses
Age of examinees: 12.3–17.8 years
Study group: pregnant adolescents
22 participants (64%) had normal or nonspecific examination findings; 8 (22%) had inconclusive findings; 4 (8%) had suggestive findings; 2 (6%) had findings of definite evidence of penetrating trauma
Heger et al. 2002 
5 year prospective study at one center in the United States (n = 2384)
Examiner(s): 2 physicians
Age of examinees: 3 months-14 years
Study group: females who reported vaginal penetration (n = 957)
Abnormal examinations were reported in only 6% of females who reported vaginal penetration
Adams et al. 1994 
Retrospective case review at one center in the United States (n = 236)
Examiner(s): 1 staff of child abuse program
Age of examinees: 8 months-17 years and 11 months
Study group: girls who reported vaginal penetration/contact (n = 213)
Normal genital exam found in 59 cases (28%), non-specific exam in 104 cases (49%), and suspicious exam in 20 cases (9%)
Size of hymenal opening of study group was 7.7 ± 2.6 mm and compared to published data on non-abused children of the same age 6.9 ± 2.2 mm
Berenson et al. 2002 
Case-control study at two centers in the United States (n = 386)
Examiner(s): 1–2 physicians
Age of examinees: 3–8 years
Group 1: controls (n = 197)
Group 2: prepubertal females with history of penile or digital penetration (n = 189)
Group 2 had larger mean transverse hymen diameter than Group 1 when examined in the knee chest position but not supine position Hymenal orifice also increased with age
No significant differences found between groups in size of vertical diameter, amount of tissue present inferiorly or laterally, or symmetry of hymen in either position
Heppenstall-Heger et al. 2003 
Prospective 10-year study at one center in United States (n = 94)
Examiner(s): three pediatricians and three nurse practitioners
Age of examinees: mean age 69.56 months (age range not specified)
Study group: 75 female children with history of vaginal penetration or trauma
Hymenal injuries were found in 37 (49.3%) of 75 girls with history of vaginal penetration or trauma
15 girls (20%) persisted with significant genital findings (i.e., a transection of the hymen)
In 80%, there was no hymenal irregularity
McCann et al. 2007 
Retrospective case review at multiple centers in the United States (n = 239)
Examiner(s): 1 physician and 2 nurses
Age of examinees: 4 months-18 years
Group 1: 113 prepubertal girls with history of vaginal penetration
Group 2: 126 pubertal adolescents with history of vaginal penetration
The hymenal injuries in Group 1 and Group 2 all healed rapidly and frequently left little or no evidence of the previous trauma
Underhill et al. 1978 
Case study at one center in the United States (n = 28)
Examiner(s): 1–2 physicians
Age of examinees: 15–48 years
Study group: self-declared virgin females
Examination confirmed virginity in 58%, was inconclusive in 11% and unconfirmed in 31% of cases
Frank et al. 1999 
Survey at one center in Turkey (n = 118)
Examiner(s): forensic physicians
Age of examinees: not specified
Study group: forensic physicians
66% of respondents reported that their findings from at least one virginity examination contradicted a recent virginity examination of the same patient
Dubow et al. 2005 
Survey at one center in United States (n = 137)
Examiner(s): pediatric chief residents
Age of examinees: Not specified
Study group: pediatric chief residents
64% correctly identified prepubertal hymen
Summary of included studies reporting on impact on examinee
Study design and population
Quality of evidence
Leclerc-Madlala S 2003 
Focus group interview in Durban, South Africa (n = 14)
Examiner(s): elderly women
Age of examinees: 13–18 years
Study group: girls planning to attend upcoming virginity testing event
Girls reported fear that being “certified” a virgin would result in brothers, friends, or neighbors raping them
Those who fail virginity tests are often expected to pay a fine for tainting the community and are excluded from certain employment
Shalhoub-Kevorkian, N 2005 
Interviews and focus groups in Jordan and Palestine (n = 41)
Examiner(s): forensic medical doctors
Age of Examinees: 21 years and younger
Study group: 7 sexually assaulted women who had virginity testing, 17 police officers, 2 physicians, 7 prosecutors, 4 social workers, and 4 lawyers
5 of 7 interviewees described the harsh trauma and aftermath of the initial sexual assault and virginity exam afterward
Focus group meetings showed women were extremely fearful and felt terrorized by virginity testing
Robatjazi et al. 2015 
In-depth semi-structured interviews in Iran (n = 15)
Examiner(s): physicians and midwives
Age of Examinees: not specified
Study group: 11 physicians and 4 midwives who performed virginity tests
10 out of 11 physicians reported that virginity testing leads to psychological distress
Most participants defined the following consequences of virginity testing: rejection, suicide, depression, weakened self-confidence, run-outs, divorce, and increased risk of diversion and abuse of girls
Frank et al. 1999 
Survey at one center in Turkey (n = 118)
Examiner: forensic physicians
Age of examinees: not specified
Study group: forensic physicians
93% responded that virginity tests are psychologically traumatic for the patient, 64% believed they were a violation of privacy, and 60% believed they result in loss of examinee’s self-esteem
Human Rights Watch 2010 
Interviews in Delhi and Mumbai, India (n = 44)
Examiner(s): gynecologists and forensic doctors
Age of examinees: not specified
Study group: direct contacts with virginity testing examinees including doctors, health rights activists, prosecutors, lawyers, and parents
The report documented the fear and re-traumatization of virginity testing on a rape victim
Doctors were reported to have harmed the examinee during the test by aggravating existing injuries
Human Rights Watch 2001 
Interviews at eight public schools in three provinces of South Africa (n = 36)
Examiner(s): Teachers and older women
Age of examinees: 7–17 years
Study group: girls who reported sexual violence at school, as well as teachers and counselors
Reported on the fear that a failed test will increase risk of abuse and discrimination
In one case, a girl's relatives broke both her arms after she failed a virginity test
Gursoy E, Vural G 2003 
Survey in eight hospitals in Ankara, Turkey (n = 101)
Examiner(s): nurses and midwives
Age of examinees: not specified
Study group: nurses and midwives
90% opposed and 10% supported virginity testing
62% agreed that a forced virginity exam might result in severe negative effects such as anxiety, depression, isolation from society, a dysfunctional sex life, guilt, worsened self-respect, and fear of death
Leclerc-Madlala S. 2001 
Observation, interviews, and focus groups in Durban, South Africa
(sample size not specified)
Examiner(s): elderly female relatives
Age of examinees: 5–22 years
Study group: key informants in virginity testing movement
Those who failed a virginity test were subject to name-calling and social exclusion
Certified non-virgins were socially excluded, reporting that they will “spoil the bunch” and “cause the flowers of the nation to wilt”
Themes of impact on the examinee
Physical harm: Virginity tests resulted in physical harm to examinees. Reported incidents include injury caused by examiner, relatives, and examinee herself. Reports include examiner-induced aggravation of existing injuries, a failed test resulting in a relative breaking examinee’s arms, and completed suicide [6, 14, 32, 33].
Psychological harm: The psychological trauma of anticipating, experiencing, and recalling the virginity test was reported by examinees and witnesses. Included are reports of extreme fear and anxiety before the test, screaming, crying, and fainting during the test, and long-term effects of self-hatred, loss of self-esteem, violation of privacy, and re-victimization of previous sexual assault [6, 14, 30, 32, 34].
Social harm: The social effects of virginity testing were documented. Included are reports of a negative test bringing shame and dishonor to families and communities, social ostracism through marriage ineligibility and exclusion from jobs, and humiliation through name-calling. Positive tests also are reported to increase a virgin’s risk of sexual assault [4, 6, 35].
Ten studies reported on the medical relevance of hymen examination as a method to determine history of vaginal intercourse, the most common type of virginity testing . The study characteristics and key findings are summarized in Table 1 [22–31]. The available research on this topic comes chiefly from physician examination of prepubertal and adolescent girls after sexual abuse allegations to determine if evidence of vaginal penetration existed. Seven of the included papers studied the accuracy of abnormal genital examinations as an indicator for history of vaginal penetration [22–28]. Abnormal genital exams included findings such as a hymenal transection, laceration, enlarged opening, or scar. Two studies reviewed physician’s accuracy in determination of virginity by exam [29, 30], and one study reported on pediatric chief residents’ ability to correctly identify the hymen by examination .
In a case-control study by Berenson et al. in the United States, genital features were compared between 192 girls with a history of vaginal penetration from sexual abuse and 200 who denied past penetration . Presence or absence of 21 different hymenal or vulvar features was compared between the two groups, such as presence of hymenal tissue, transections, perforations, and notches. It was found that only 2.5% of physical exam findings were unique to the group with a history of penetration.
Kellog et al. studied a cohort in the United States with definitive evidence of previous vaginal penetration. In the study of 26 pregnant adolescents who reported sexual abuse, 22 participants (64%) had normal or nonspecific genital examination findings, eight (22%) had inconclusive findings, four (8%) had suggestive findings, and two (6%) had findings of definite evidence of vaginal penetration .
In one large cohort of 2384 children in the United States, 957 girls reported penetration from sexual abuse. Of these 957 girls, only 61 (6%) had abnormal genital examination findings . The study’s parameters for abnormal examination included: “acute trauma, transections of the hymen that extended to the base of the hymen, scarring, sexually transmitted diseases, and positive forensics” .
In a retrospective case review in the United States of 213 girls who reported vaginal penetration/contact from sexual abuse, there was a normal genital exam in 59 cases (28%), non-specific findings in 104 cases (49%), and suspicious findings in 20 cases (9%) . Size of hymenal opening was also measured on the study group (7.7 ± 2.6 mm) and compared to published data on non-abused children of the same age (6.9 ± 2.2 mm), and no significant difference was found in mean size .
Hymenal opening size was measured in a United States case-control study of 189 girls with, and 197 girls without, a history of penile or digital penetration from sexual abuse . The former group had a larger mean transverse hymen diameter than the latter when examined in the knee-chest position but not supine position; hymenal orifice size also increased with age.
Healing of injuries to the hymen was reviewed in two studies, both in the contexts of sexual abuse allegations in the United States [27, 28]. In a study of 75 girls with a history of a vaginal penetration or trauma, injuries to the hymen were found in 37 cases (49.3%), significant genital findings (i.e., a transection of the hymen) were found in 15 girls (20%), and in the remaining 80%, there was no increase in the hymenal diameter or irregularity or narrowing of the hymen . In a study of 113 prepubertal girls and 126 pubertal girls with previous penetration that reported on healing of injuries to the hymen, it was found that hymenal injuries in both groups healed rapidly and often left little or no evidence of previous trauma .
A 1978 study in the United States reported on the accuracy of physicians in confirming virginity through hymen examination . Two gynecologists inspected the hymens of a cohort of 28 self-declared virgins. The physicians reported that examination of the hymen confirmed virginity in 16 cases (58%), was inconclusive in nine cases (31%), and uncertain in three cases (11%) .
In a study of forensic physicians in Turkey, 66% of respondents reported that their findings from at least one virginity exam contradicted a recent virginity exam of the same patient .
Lastly, a study examined physician knowledge of hymen anatomy . In 2005, 137 United States pediatric chief residents were asked to identify the hymen on a photograph of pediatric anatomy; 64% were able to correctly identify the structure .
Quality of the ten studies reporting on the medical relevance of virginity testing was assessed according to USPSTF guidelines and is reported in Table 1. (Additional information regarding the USPSTF grading criteria is provided in the Additional file 1). The level of evidence ranged from level II-2 to level III. Seven studies were level II-2 [22–28], one study was level II-3 , and two studies were level III [30, 31]. The internal validity of the studies reporting on medical relevance ranged from good to poor. Two studies had good internal validity [22, 26], six had fair internal validity [24, 25, 27, 28, 30, 31], and two had poor interval validity [23, 29].
Impact on examinee
Eight studies provided evidence on the effects of virginity testing on the examinee [4, 6, 14, 30, 32–35]. The study characteristics and key findings are summarized in Table 2. Included studies provided data on the experiences of the examinee and those who worked directly with the examinee (such as doctors, social workers, police officers, and lawyers). Six studies [4, 6, 14, 32, 33, 35] provided data from interviews and focus group discussions and two studies [30, 34] provided survey data from healthcare professionals. Three themes were constructed from the data: physical harm, psychological harm, and social harm. Themes are presented in Table 3, and expanded on below with relevant study findings and rationale of theme selection.
Physical harm of virginity examinees was reported in four studies [6, 14, 32, 33]. In a study of virginity testing in Palestine, a social worker present during her client’s virginity exam reported that, “the process was very painful, she was crying, screaming, holding my hands” . Undergoing virginity exams also caused two of the social worker’s clients to become suicidal, with one reported attempted suicide . When one examinee failed her virginity test, she was told that she would need to, “search for a way to save herself from the deadly consequences that awaited her” . In a report of virginity testing in Iran, one medical examiner reported an exam that lead to death, “I told her that her hymen was not intact, and she said that she had done nothing. Then I heard she had committed suicide” . A report on India’s two-finger test describes doctors who harmed the examinee during the test by aggravating existing injuries . In South Africa, a report was made to Childline, a helpline that offers rape counseling, of an examinee’s relatives breaking both of her arms after she failed a virginity test .
extremely fearful of and indeed felt terrorized by [the experience]. … Their feelings of fear and invasion were manifested in a variety of ways: by their refusal to sit on the examination chair, through crying, screaming, pushing, freezing-up, being silent, fainting, etc. 
One social worker described virginity exams as torture: “… I also felt it is so unfair to be sexually abused and then [have to] go through such a vicious process of torture” . In depth interviews of medical professionals who performed virginity testing in Iran revealed that the virginity test resulted in the psychological distress of the examinee, causing “rejection, suicide, depression, and weakened self-confidence” . A survey of forensic physicians in Turkey found that 93% of 118 respondents agreed that virginity tests are psychologically traumatic for the patient, 64% believed they were a violation of privacy, and 60% believed they result in loss of examinee’s self-esteem . Interviews from a report on India’s two-finger test documented the fear and re-traumatization the examination causes . In a study of 101 nurses and midwives in Turkey, 90% indicated they were opposed to virginity testing, and when asked why, nearly half agreed that they were opposed because the examinations are being done against the examinee’s will . Sixty-two percent of the nurses and midwives also agreed that a forced virginity exam may result in severe negative effects such as anxiety, depression, isolation from society, a dysfunctional sex life, guilt, worsened self-respect, and fear of death .
Social harm was reported in three studies [4, 6, 35]. Leclerc-Madlala reported in a study in South Africa that those who fail virginity tests are often expected to pay a fine for tainting the community . They are also excluded from certain employment opportunities, illustrated by one factory owner who stated that her various franchises throughout KwaZulu-Natal and the Eastern Cape had tested and selectively employed over 4000 virgins, which she believed was a service to the community and state . Social harms of positive virginity tests were also noted. In a focus group interview of 14 girls who were planning to attend a virginity testing event, the girls reported that their primary concern was that being “certified” a virgin would result in brothers, friends, or neighbors raping them. They spoke of previous cases in which this had occurred in their community. Rape in this context was reported most likely to occur as a gang rape by several boys who needed to “teach her a lesson” and show her “what men are all about” . A study in Palestine detailed one examinee’s fear of adverse social consequences . She was afraid that a failed virginity test would result in loss of honor and social condemnation. The examinee stated, “I wanted to do [the examination]. I wanted to know if I lost my honor. I paid to learn that I lost my honor” . In another study of South Africa’s KwaZulu-Natal province by Leclerc-Madlala, those who failed a virginity test were subject to name-calling and social exclusion . One respondent referred to a girl who failed as a "rotten potato" who must be kept away from the ‘virgin girls’ because she will surely “spoil the bunch.” Another respondent noted that being in close proximity to a girl who failed the test would, “cause the flowers of the nation to wilt” .
Quality of the eight studies reporting on the impact of virginity testing on examinee was assessed according to USPSTF guidelines and is reported in Table 2. All eight studies were level III evidence [4, 6, 14, 30, 32–35]. The internal validity of the studies reporting on impact on examinee ranged from fair to poor. Four studies had fair internal validity [6, 14, 30, 34] and four had poor internal validity [4, 32, 33, 35].
The present review assessed 17 published studies on virginity testing, in particular its medical relevance and impact on the examinee.
The utility of hymen examination as a test for virginity was reviewed [22–31]. The studies indicated, as has been described in previous reviews, that the inspection of the hymen cannot give conclusive evidence of vaginal penetration or any other sexual history [36, 37]. Normal hymen examination findings are likely to occur in those with and without a history of vaginal penetration [22–30]. A hymen exam with abnormal findings is also inconclusive: abnormal hymenal features such a hymenal transection, laceration, enlarged opening, or scars are found in females with and without a history of sexual intercourse [22–30].
One hymenal feature commonly examined in virginity testing is hymenal opening size. Hymenal opening size also was found to be an unreliable test for vaginal penetration [25–27]. Hymen opening size varies with the method of examination, the position of the examinee, the cooperation and relaxation of the examinee, and the examinee’s age, weight, and height . With regards to healing of hymenal injuries, it was found that most hymenal injuries heal rapidly and leave no evidence of previous trauma [26, 27].
Six studies reporting on medical utility included in the present review were limited by not having a control group [23–25, 27–29]. Studies without control groups make it difficult to interpret whether exam findings were accurately labeled as indicative or suspicious of vaginal penetration, as limited information is known about the appearance of the hymen after injury . Another limitation is that most studies that reported on the medical utility of hymen examination were performed on females from the United States to determine if evidence existed after sexual abuse allegations, whereas most routine use of virginity tests has been reported outside of the United States and for the purpose of assessing moral or social value [1, 2]. The lack of data from outside the United States may affect the generalizability of results to females examined elsewhere. The ages of examinees of included studies were heterogeneous, with reported ages varying from 3 months to 48 years. Four studies combined females from different ages and stages of development [24, 25, 28, 29]. The heterogeneity in age groups may have caused observed differences to be due to differences in age, as it is known that hymenal features vary with age [23–25, 27–29]. Lastly, heterogeneity exists in regards to the knowledge and experience of examiner.
The search carried out for this review was comprehensive but did not include unpublished studies or studies in languages other than English, and thus there is potential to have missed relevant studies in other languages.
Another form of virginity testing is performed by insertion of two fingers into the vagina to examine its laxity . This form of virginity testing was not included in the review of literature because the medical community has not considered vaginal laxity a clinical indicator of previous sexual intercourse. The vagina is a dynamic muscular canal that varies in size and shape depending on individual, developmental stage, physical position, and various hormonal factors such as sexual arousal and stress [38, 39]. However, there are reports that the so-called ‘finger testing’ has been used in countries like India to assess evidence for sexual assault . It can also be found in forensic examination forms in some countries.
Studies of the effects of virginity testing on the examinee are also limited. Eight studies on the effects of virginity testing were identified and reviewed [4, 6, 14, 30, 32–35]. The review found that the virginity exam itself had resulted in physical harm of the examinee. This is supported by news reports from Turkey in which five by students attempted suicide by consuming rat poison to avoid undergoing the virginity test . Virginity exams also commonly resulted in psychological trauma with long-lasting adverse effects, including but not limited to anxiety, depression, loss of self-esteem, and suicidal ideation. Health professionals also identified violation of privacy and autonomy as adverse effects [6, 34]. Lastly, virginity exams were reported to have an adverse social impact including social exclusion, perceived dishonor brought to family and community, employment discrimination, humiliation, and increased risk of sexual assault [4, 6, 35]. More research is needed to understand better the short and long term consequences of the virginity exam on the examinee.
This review found that virginity examination, also known as two-finger, hymen, or per-vaginal examination, is not a useful clinical tool, and can be physically, psychologically, and socially devastating to the examinee. From a human rights perspective, virginity testing is a form of gender discrimination, as well as a violation of fundamental rights, and when carried out without consent, a form of sexual assault.
A gap exists between current medical evidence of virginity testing and medical education and training [9, 30, 33, 35]. Some forensic medical textbooks still include virginity testing as a standard procedure for assessment of sexual assault [38, 40–42]. Medical schools and public health professionals must update their textbooks, courses, and training to eliminate any recommendations of virginity testing, and educate others on the lack of scientific evidence for and possible harms of its use.
Governments, medical establishments, and health professional associations in all countries, even those with no history of virginity testing, should take the initiative to ban the use of virginity testing and create national guidelines for health professionals, public officials, and community leaders. More research is urgently needed to understand the regional and cultural rationales for virginity testing, and to develop more robust and efficacious education strategies that involve communities.
This work was undertaken while RO was an intern in the Department of Reproductive Health and Research of the World Health Organization. No specific funding was received for this work.
Availability of data and materials
All data generated or analysed during this study are included in this published article (and its Additional file 1).
CGM conceptualized and oversaw the review. RO carried out the search and data extraction and CGM reviewed the search and data extraction. Both authors were involved in writing the paper based on first draft of the report prepared by RO with input from CGM. Both authors reviewed and agreed on the final manuscript.
RO attends the University of Minnesota Medical School. She is a Duke University Global Health Fellow, and interned with the Reproductive Health and Research Department at the WHO under CGM. CGM coordinates the work on violence against women at the World Health Organization, Department of Reproductive Health and Research. She is a physician with an MSc in Community Medicine and has led research, policy and guidelines development work on women’s health, health and gender equality and violence against women.
The authors declare that they have no competing interests. The views expressed here are solely the responsibility of the authors and do not necessarily represent the views or policy of the World Health Organization.
Consent for publication
Ethics approval and consent to participate
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Independent Forensic Expert Group. Statement on virginity testing. J Forensic Leg Med. 2015;33:121–4.View ArticleGoogle Scholar
- Khambati N. India's two finger test after rape violates women and should be eliminated from medical practice. BMJ. 2014;348:g3336–g36.View ArticlePubMedGoogle Scholar
- Behrens K. Virginity testing in South Africa: a cultural concession taken too far? South African Journal of Philosophy. 2014;33(2):177–87.View ArticleGoogle Scholar
- Leclerc-Madlala S. Protecting girlhood? Virginity revivals in the era of AIDS. Agenda: Empowering Women for Gender Equality. 2003;56:16–25.Google Scholar
- Egypt women protesters forced to take 'virginity tests'. BBC. 24 March 2014. http://www.bbc.com/news/world-middle-east-12854391. Accessed 5 Aug 2015.
- Shalhoub-Kevorkian N. Imposition of virginity testing: a life-saver or a license to kill? Soc Sci Med. 2005;60(6):1187–96.View ArticlePubMedGoogle Scholar
- Amnesty International. Stop Violence Against Women: It's In Our Hands. London: Amnesty International Publications; 2004. p. 18. https://www.amnesty.ie/wp-content/uploads/2016/05/Its-in-our-Hands.pdf. Accessed 5 Aug 2015.
- Percy J. Love crimes: what liberation looks like for Afghan women. Harper's Magazine. 2015:1–18. http://harpers.org/archive/2015/01/love-crimes/. Accessed 28 July 2015
- Ayotte B. State-control of female virginity in Turkey: the role of physicians. Journal of Ambulatory Care Management. 2000;23(1):89–91.View ArticlePubMedGoogle Scholar
- Bangladesh: Court Prohibits Use of the "Two-Finger Test" [website]. Avon Global Center for Women and Justice, Cornell Law School; 2015. http://www.lawschool.cornell.edu/womenandjustice/Featured-Cases/Bangladesh-Court-Prohibits-Use-of-the-Two-Finger-Test.cfm. Accessed 15 July 2015.
- Jayaweera S, Sanmugam T. Impact of macro economic reforms on women in Sri Lanka. Colombo: Centre for Women's Research; 2001.Google Scholar
- Kine P. Indonesia ‘Virginity Tests’ Run Amok. Human Rights Watch. 9 February 2015. https://www.hrw.org/news/2015/02/09/dispatches-indonesia-virginity-tests-run-amok. Accessed 10 Aug 2015.
- Human Rights Watch. ‘Virginity Tests’ for Female Police in Indonesia. https://www.hrw.org/video-photos/video/2014/11/17/virginity-tests-female-police-indonesia. Accessed 10 Aug 2015.
- Robatjazi M, Simbar M, Nahidi F, Gharehdaghi J, Emamhadi M, Vedadhir AA, Alavimajd H. Virginity Testing Beyond a Medical Examination. Glob J Health Sci. 2015;18;8(7):152–64.View ArticleGoogle Scholar
- Behrens K. Why physicians ought not to perform virginity tests. J Med Ethics. 2015;41(8):691–5.View ArticlePubMedGoogle Scholar
- United Nations. Transforming our world: the 2030 Agenda for Sustainable Development. New York: United Nations; 2015.Google Scholar
- Moher D, Liberati A, Tetzlaff J, Altman DG, PRISMA Group. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS Med. 2009;6(7):e1000097.View ArticlePubMedPubMed CentralGoogle Scholar
- Higgins J, Green S. Cochrane handbook for systematic reviews of interventions. Chichester: Wiley-Blackwell; 2008.View ArticleGoogle Scholar
- Harris RP, Helfand M, Woolf SH, Lohr KN, Mulrow CD, Teutsch SM, et al. Current methods of the US Preventive Services Task Force: a review of the process. Am J Prev Med. 2001;20(3 Suppl):21–35.View ArticlePubMedGoogle Scholar
- US Preventive Services Task Force. Guide to Clinical Preventive Services: Report of the U.S. Preventive Services Task Force. 2nd ed. Baltimore: Williams & Wilkins; 1996.Google Scholar
- Yeaton WH, Lagenbrunner JC, Smyth JM, Wortman PM. Exploratory research synthesis--methodological considerations for addressing limitations in data quality. Eval Health Prof. 1995;18(3):283.View ArticlePubMedGoogle Scholar
- Berenson A, Chacko M, Wiemann C, Mishaw C, Friedrich W, Grady J. A case–control study of anatomic changes resulting from sexual abuse. Am J Obstet Gynecol. 2000;182(4):820–34.View ArticlePubMedGoogle Scholar
- Kellogg N, Menard S, Santos A. Genital anatomy in pregnant adolescents: "Normal" does not mean "Nothing Happened". PEDIATRICS. 2003;113(1):e67–9.View ArticleGoogle Scholar
- Heger A, Ticson L, Velasquez O, Bernier R. Children referred for possible sexual abuse: medical findings in 2384 children. Child Abuse Negl. 2002;26(6–7):645–59.View ArticlePubMedGoogle Scholar
- Adams J, Harper K, Knudson S, Revilla J. Examination findings in legally confirmed child sexual abuse: it's normal to be normal. PEDIATRICS. 1994;94(3):310–3.PubMedGoogle Scholar
- Berenson A, Chacko M, Wiemann C, Mishaw C, Friedrich W, Grady J. Use of hymenal measurements in the diagnosis of previous penetration. PEDIATRICS. 2002;109(2):228–35.View ArticlePubMedGoogle Scholar
- Heppenstall-Heger A, McConnell G, Ticson L, Guerra L, Lister J, Zaragoza T. Healing patterns in anogenital injuries: a longitudinal study of injuries associated with sexual abuse, accidental injuries, or genital surgery in the preadolescent child. PEDIATRICS. 2003;112(4):829–37.View ArticlePubMedGoogle Scholar
- McCann J, Miyamoto S, Boyle C, Rogers K. Healing of hymenal injuries in prepubertal and adolescent girls: a descriptive study. PEDIATRICS. 2007;119(5):e1094–106.View ArticlePubMedGoogle Scholar
- Underhill R, Dewhurst J. The doctor cannot always tell. Medical examination of the "intact" hymen. Lancet. 1978;1(8060):375–6.View ArticlePubMedGoogle Scholar
- Frank M, Bauer H, Arican N, Korur Fincanci S, Iacopino V. Virginity examinations in Turkey. JAMA. 1999;282(5):485.View ArticlePubMedGoogle Scholar
- Dubow S, Giardino A, Christian C, Johnson C. Do pediatric chief residents recognize details of prepubertal female genital anatomy: a national survey. Child Abuse Negl. 2005;29(2):195–205.View ArticlePubMedGoogle Scholar
- Dignity on Trial. New York City: Human Rights Watch; 2010. http://www.hrw.org/sites/default/files/reports/india0910webwcover.pdf. Accessed 19 July 2015.
- Scared At School. New York City: Human Rights Watch: 2001. http://www.hrw.org/legacy/reports/2001/safrica/. Accessed 21 July 2015
- Gursoy E, Vural G. Nurses' and midwives' views on approaches to hymen examination. Nurs Ethics. 2003;10(5):485–96.View ArticlePubMedGoogle Scholar
- Leclerc-Madlala S. Virginity testing: managing sexuality in a maturing HIV/AIDS epidemic. Med Anthropol Q. 2001;15(4):533–52.View ArticlePubMedGoogle Scholar
- Berkoff MC, Zolotor AJ, Makoroff KL, Thackeray JD, Shapiro RA, Runyan DK. Has this prepubertal girl been sexually abused? JAMA. 2008;300(23):2779–92.View ArticlePubMedGoogle Scholar
- Pillaim M. Genital findings in prepubertal girls: what can be concluded from an examination? J Pediatr Adolesc Gynecol. 2008;21(4):177–85.View ArticleGoogle Scholar
- Padubidri V, Daftary S. Shaw's Textbook of Gynecology. 16th ed. New Delhi: Elsevier Health Sciences APAC; 2014.Google Scholar
- Lloyd J, Crouch N, Minto C, Liao L, Creighton S. Female genital appearance: ‘normality’ unfolds. BJOG. 2005;112(5):643–6.View ArticlePubMedGoogle Scholar
- Subrahmanyam B, Modi J. Modi's medical jurisprudence and toxicology. 22nd ed. New Delhi: Butterworths India; 2001.Google Scholar
- Parikh C. Parikh's textbook of medical jurisprudence and toxicology. 6th ed. New Delhi: CBS Publishers and Distributor; 2005.Google Scholar
- Narayan Reddy K. The essentials of forensic medicine and toxicology. 26th ed. Hyderabad: K. Suguna Devi; 2007.Google Scholar